2QF3
Structure of the delta PDZ truncation of the DegS protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 125 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-05-11 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.347, 75.316, 162.257 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.610 - 2.040 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qf0 chains A B C |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.067 | 0.468 |
| Number of reflections | 56302 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 98.1 | 83.8 |
| Redundancy | 6.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 293 | 0.2 M ammonium phosphate (monobasic), 0.1 M Tris pH 8.5, 30% methylpentanediol (MPD), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
