2QCA
A New Crystal Form of Bovine Pancreatic RNase A in Complex with 2'-Deoxyguanosine-5'-monophosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 2006-01-08 |
Detector | RIGAKU RAXIS |
Spacegroup name | P 31 |
Unit cell lengths | 55.080, 55.080, 39.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.540 - 1.330 |
R-factor | 0.1253 |
Rwork | 0.119 |
R-free | 0.17710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rno |
RMSD bond length | 0.012 |
RMSD bond angle | 0.032 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.540 | 1.380 |
High resolution limit [Å] | 1.330 | 1.330 |
Rmerge | 0.049 | 0.491 |
Number of reflections | 27155 | |
<I/σ(I)> | 13.7 | 0.8 |
Completeness [%] | 89.3 | 24.9 |
Redundancy | 3.33 | 1.49 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 295 | Reservoir: 0.6 ml of 30% PEG 3350 in 0.1 M HEPES at pH 7.0. Sample: 2 ul 10-40 mg/ml Protein in 0.1 M HEPES at pH 7.0 and 2 ul of 0.1 M DGP, cholesterol, thymine and oxamic acid in 15% PEG 3350 and 9.1 M HEPES at pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K, pH 7.00 |