2Q9O
Near-atomic resolution structure of a Melanocarpus albomyces laccase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.93100 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 173.740, 61.900, 124.210 |
Unit cell angles | 90.00, 96.36, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.300 |
R-factor | 0.1674 |
Rwork | 0.167 |
R-free | 0.21240 |
Structure solution method | AB INITIO |
RMSD bond length | 0.010 |
RMSD bond angle | 0.028 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | SHELX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.400 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 310642 | |
<I/σ(I)> | 11.4 | 3 |
Completeness [%] | 96.7 | 91.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 295 | 15% PMME 2000, 0.2 M ammonium sulphate, 0.1 M sodium acetate , pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |