2Q66
Structure of Yeast Poly(A) Polymerase with ATP and oligo(A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0809 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.633, 85.907, 107.469 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.710 - 1.800 |
R-factor | 0.191 |
Rwork | 0.189 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 2HHP |
RMSD bond length | 0.010 |
RMSD bond angle | 1.244 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.860 |
High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
Rmerge | 0.072 | 0.049 | 0.275 |
Number of reflections | 56633 | ||
<I/σ(I)> | 12.8 | ||
Completeness [%] | 95.7 | 99.9 | 76 |
Redundancy | 12 | 12 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 273 | .1M bis tris propane pH 6.4, .2M Li acetate, 16% Peg 3350, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | bis tris propane | ||
2 | 1 | 1 | Li acetate | ||
3 | 1 | 1 | Peg | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | bis tris propane | ||
6 | 1 | 2 | Li acetate | ||
7 | 1 | 2 | Peg |