2PS0
Structure and metal binding properties of ZnuA, a periplasmic zinc transporter from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-03-03 |
Detector | MAR scanner 300 mm plate |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.438, 86.266, 88.147 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.200 - 2.000 |
R-factor | 0.201 |
Rwork | 0.199 |
R-free | 0.25000 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.188 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.200 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 38461 | |
Completeness [%] | 99.8 | 98.4 |
Redundancy | 6.8 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 293 | 30 % (w/v) PEG 4000, 5 % isopropanol, 0.1 M succinate, pH 4.9 mixed 1:1 with 10 mg/mL ZnZnuA or 1:2 with 2.5 mg/mL ZnZnuA, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 293 | 30 % (w/v) PEG 4000, 5 % isopropanol, 0.1 M succinate, pH 4.9 mixed 1:1 with 10 mg/mL ZnZnuA or 1:2 with 2.5 mg/mL ZnZnuA, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 293 | 30 % (w/v) PEG 4000, 5 % isopropanol, 0.1 M succinate, pH 4.9 mixed 1:1 with 10 mg/mL ZnZnuA or 1:2 with 2.5 mg/mL ZnZnuA, VAPOR DIFFUSION, HANGING DROP, temperature 293K |