2PQI

Crystal structure of active ribosome inactivating protein from maize (b-32)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-11-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54178
Spacegroup name	P 32
Unit cell lengths	115.190, 115.190, 45.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.2172
Rwork	0.217
R-free	0.26140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pqg
RMSD bond length	0.008
RMSD bond angle	1.449
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.031	0.153
Number of reflections	21654
<I/σ(I)>	22.2	4.1
Completeness [%]	93.9	90.1
Redundancy	2.4	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	299	0.2 M Sodium acetate tri-hydrate, 0.1 M Tris-HCl pH 8.5, 30% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 299K