2PH0
Crystal structure of the Q6D2T7_ERWCT protein from Erwinia carotovora. NESG target EwR41.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-30 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97960 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 132.584, 38.736, 58.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.760 - 1.850 |
R-factor | 0.219 |
Rwork | 0.219 |
R-free | 0.24300 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.056 | 0.451 |
Number of reflections | 49308 | |
<I/σ(I)> | 22.38 | 3.9 |
Completeness [%] | 99.1 | 97.8 |
Redundancy | 7 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7 | 291 | 40% PEG 1000, 0.1M Ammonium nitrate, 0.1M MOPS, pH 7.0, Microbatch, temperature 291K |