2PFS
Crystal structure of universal stress protein from Nitrosomonas europaea
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 76.000, 76.000, 42.987 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.250 |
R-factor | 0.19758 |
Rwork | 0.195 |
R-free | 0.25455 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.563 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.290 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.060 | 0.486 |
Number of reflections | 6882 | |
<I/σ(I)> | 58.5 | 2.9 |
Completeness [%] | 97.6 | 81.5 |
Redundancy | 11.1 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 0.2M Sodium chloride, 0.1M HEPES pH 7.5, 25% PEG3350, 2% Isopropanol, VAPOR DIFFUSION, temperature 293K |