2PFL
CRYSTAL STRUCTURE OF PFL FROM E.COLI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-02 |
Detector | MARRESEARCH |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 159.020, 159.020, 160.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.900 |
Rwork | 0.202 |
R-free | 0.24240 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 22.100 * |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.900 |
Rmerge | 0.088 |
Total number of observations | 186926 * |
Number of reflections | 45502 |
Completeness [%] | 98.4 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 7.3 | pH 7.3 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
10 | 1 | reservoir | EDTA | 1 (mM) | |
11 | 1 | reservoir | 3 (mM) | ||
12 | 1 | reservoir | 2 (mM) | ||
13 | 1 | reservoir | PEG1000 | 24-30 (%(w/v)) | |
2 | 1 | drop | Mops/NH3 | 25 (mM) | |
3 | 1 | drop | 50 (mM) | ||
4 | 1 | drop | dithiothreitol | 10 (mM) | |
5 | 1 | drop | 3 (mM) | ||
6 | 1 | drop | PEG1000 | 16 (%(w/v)) | |
7 | 1 | reservoir | Mops/NH3 | 25 (mM) | |
8 | 1 | reservoir | 50 (mM) | ||
9 | 1 | reservoir | dithiothreitol | 1 (mM) |