2P7P
Crystal structure of genomically encoded fosfomycin resistance protein, FosX, from Listeria monocytogenes complexed with MN(II) and sulfate ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-12-05 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 169.500, 70.565, 84.145 |
Unit cell angles | 90.00, 113.74, 90.00 |
Refinement procedure
Resolution | 23.080 - 2.170 |
Rwork | 0.226 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2p7k |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.250 |
High resolution limit [Å] | 2.170 | 2.170 |
Number of reflections | 46644 | |
<I/σ(I)> | 14.7 | 2.1 |
Completeness [%] | 97.1 | 85.8 |
Redundancy | 2.1 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 30% PEG 8000, 50 mM Na cacodylate, 0.1 M (NH4)2SO4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |