2P3Y
Crystal structure of VPA0735 from Vibrio parahaemolyticus. NorthEast Structural Genomics target VpR109
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-20 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97900 |
Spacegroup name | P 1 |
Unit cell lengths | 61.764, 64.253, 78.524 |
Unit cell angles | 84.81, 89.14, 76.47 |
Refinement procedure
Resolution | 35.700 - 1.800 |
R-factor | 0.204 |
Rwork | 0.204 |
R-free | 0.23400 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.027 | 0.121 |
Number of reflections | 194335 | |
<I/σ(I)> | 27.5 | 24.6 |
Completeness [%] | 89.4 | 95.6 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 200mM MOPS, PEG8000, MgCl2, 10mM DTT, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |