2OZY
Crystal structure of E.coli nrfB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-05 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.683, 59.668, 65.207 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.020 - 1.740 |
R-factor | 0.171 |
Rwork | 0.169 |
R-free | 0.20400 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.504 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.023 | 1.830 |
High resolution limit [Å] | 1.740 | 1.740 |
Rmerge | 0.122 | 0.451 |
Number of reflections | 19836 | |
<I/σ(I)> | 4.8 | 1.6 |
Completeness [%] | 97.4 | 99.3 |
Redundancy | 2.8 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 289 | Purified NrfB was concentrated to 8 mg/ml before crystallization. Crystals were obtained by the vapour diffusion method under anaerobic conditions using 250 mM tri-sodium citrate, 10 mM Na2S2O4 and 20 % (v/v) isopropanol in 100 mM sodium HEPES pH 7.5, temperature 289K |