2OX4
Crystal structure of putative dehydratase from Zymomonas mobilis ZM4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-09 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97960 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 190.178, 190.409, 85.922 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.14513 |
Rwork | 0.144 |
R-free | 0.18553 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gl5 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.171 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.3.0028) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.094 | 0.480 |
Number of reflections | 288410 | |
<I/σ(I)> | 4.8 | 2.2 |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 6.7 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 100mM Tris-HCl, pH 8.5, 30% PEG 4000, 200mM Lithium sulfate, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |