2OOE
Crystal structure of HAT domain of murine CstF-77
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9798 |
Spacegroup name | H 3 2 |
Unit cell lengths | 105.675, 105.675, 358.157 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.170 - 3.000 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.29400 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB (THEN SOLVE/RESOLVE) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.071 | 0.199 |
Number of reflections | 15612 | |
<I/σ(I)> | 16.1463 | 5.691 |
Completeness [%] | 98.1 | 99.4 |
Redundancy | 3.7 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 50mM Bis-tris, 50mM ammonium sulfate, 30%(v/v) pentaerythritol ethoxylate(15/4EO/OH), pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |