2OKW
A non-invasive GFP-based biosensor for mercury ions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2006-06-03 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.979454 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.178, 128.843, 147.889 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.830 - 1.900 |
Rwork | 0.232 |
R-free | 0.26790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1emb |
RMSD bond length | 0.005 |
RMSD bond angle | 1.374 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.830 |
High resolution limit [Å] | 1.900 |
Rmerge | 0.073 |
Number of reflections | 155659 |
<I/σ(I)> | 15.7 |
Completeness [%] | 99.7 |
Redundancy | 7.16 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 100mM Pipes, 30% PEG 8000, 200mM Na-acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |