2OCA
The crystal structure of T4 UvsW
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 118.640, 155.200, 101.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| Rwork | 0.217 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rif |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.261 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.082 | 0.418 |
| Number of reflections | 25580 | |
| <I/σ(I)> | 35.2 | 3.15 |
| Completeness [%] | 97.4 | 82.2 |
| Redundancy | 6.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 1.0 M Sodium Malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
