2O54
Structure of E. coli topoisomerase III in complex with an 8-base single stranded oligonucleotide. Frozen in glycerol at pH 7.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-01-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9756 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 101.830, 101.830, 452.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.103 | 2.540 |
High resolution limit [Å] | 2.479 | 2.480 |
Rmerge | 0.072 | 0.262 |
Total number of observations | 12741 | |
Number of reflections | 82671 | |
<I/σ(I)> | 8.4 | 2.9 |
Completeness [%] | 96.8 | 75.9 |
Redundancy | 4.8 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 1.5 M (NH4)SO4, 0.1 M Sodium citrate, 0.5 M NaCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | (NH4)SO4 | ||
2 | 1 | 1 | Sodium citrate | ||
3 | 1 | 1 | NaCl | ||
4 | 1 | 1 | HOH | ||
5 | 1 | 2 | (NH4)SO4 | ||
6 | 1 | 2 | Sodium citrate | ||
7 | 1 | 2 | NaCl | ||
8 | 1 | 2 | HOH |