2O3J
Structure of Caenorhabditis Elegans UDP-Glucose Dehydrogenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-15 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 159.716, 144.092, 92.072 |
Unit cell angles | 90.00, 123.24, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.880 |
R-factor | 0.2102 |
Rwork | 0.209 |
R-free | 0.26315 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dli |
RMSD bond length | 0.010 |
RMSD bond angle | 1.114 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.064 | 0.730 |
Number of reflections | 133254 | |
<I/σ(I)> | 2.7 | 0.8 |
Completeness [%] | 98.7 | 90.4 |
Redundancy | 2.7 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 290 | 200 MM MgCl2, 100 MM BIS-TRIS, PH 5.50, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K |