2O34
Crystal structure of protein DVU1097 from Desulfovibrio vulgaris Hildenborough, Pfam DUF375
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-24 |
Detector | MAR CCD 165 mm |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.480, 81.480, 151.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.980 - 1.950 |
R-factor | 0.213 |
Rwork | 0.210 |
R-free | 0.27300 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.483 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.106 | 0.674 |
Number of reflections | 55000 | |
<I/σ(I)> | 8.3 | |
Completeness [%] | 96.3 | 63.5 |
Redundancy | 4.8 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 295 | 2.0M sodium formate, 0.1M Na acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 295K, pH 4.60 |