2NQ6
Crystal structure of human methionine aminopeptidase type 1 in complex with 3-tert-Butoxycarbonylaminopyridine-2-carboxylic acid thiazole-2-ylamide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.977 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.320, 77.825, 48.229 |
Unit cell angles | 90.00, 90.48, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.500 |
Rwork | 0.173 |
R-free | 0.20350 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 2.100 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | EPMR |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.500 |
Rmerge | 0.046 |
Number of reflections | 45141 |
<I/σ(I)> | 19 |
Completeness [%] | 80.8 |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 6% PEG 10 000, 100 mM HEPES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |