2N3R

NMR structure of the II-III-VI three-way junction from the VS ribozyme and identification of magnesium-binding sites using paramagnetic relaxation enhancement

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Varian	UNITY	Varian Unity	600

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	1D 1H watergate
10	1	6	2D A-specific H(NC)-TOCSY-(C)H
11	2	2	2D 1H-15N MQ-(HC)N(C)H
12	2	2	2D 1H-13C HSQC
13	2	2	2D 1H-13C HMQC
14	2	2	3D HCCH-COSY
15	2	2	3D HCCH-TOCSY
16	2	2	3D 13C-edited NOESY-HSQC
17	2	3	2D 1H-13C HSQC
18	2	3	2D 1H-13C HMQC
19	2	3	3D HCCH-COSY
2	1	1	2D 1H-15N HSQC
20	2	3	3D HCCH-TOCSY
21	2	3	3D 13C-edited NOESY-HSQC
22	2	4	2D 1H-13C HSQC
23	2	4	2D 1H-13C HMQC
24	2	4	3D HCCH-COSY
25	2	4	3D HCCH-TOCSY
26	2	4	3D 13C-edited NOESY-HSQC
27	2	5	2D 1H-13C HSQC
28	2	5	2D 1H-13C HMQC
29	2	5	3D HCCH-COSY
3	1	1	2D 1H-15N HSQC NH2 only
30	2	5	3D HCCH-TOCSY
31	2	5	3D 13C-edited NOESY-HSQC
4	1	1	2D HNN COSY
5	1	1	2D 1H-1H NOESY
6	1	1	2D 1H-15N CPMG-NOESY
7	1	1	3D 15N-edited NOESY-HSQC
8	1	6	3D 15N/13C-edited NOESY-HSQC
9	1	6	2D G-specific H(NC)-TOCSY-(C)H

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	6.5	ambient	288
2	6.5	ambient	298

Conformers

Conformers Calculated Total Number	500
Conformers Submitted Total Number	21