2LYO
CROSS-LINKED CHICKEN LYSOZYME CRYSTAL IN 90% ACETONITRILE-WATER
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 285 |
Detector technology | IMAGE PLATE |
Collection date | 1995-12 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.230, 79.230, 37.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.930 |
R-factor | 0.178 |
Rwork | 0.178 |
R-free | 0.20300 |
Structure solution method | DIRECT REFINEMENT |
Starting model (for MR) | 6lyt |
RMSD bond length | 0.012 |
RMSD bond angle | 24.000 * |
Data reduction software | R-AXIS |
Data scaling software | R-AXIS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 80.000 |
High resolution limit [Å] | 1.930 |
Rmerge | 0.043 |
Total number of observations | 55181 * |
Number of reflections | 8855 |
<I/σ(I)> | 16.6 |
Completeness [%] | 92.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.1 * | 24-25 * | PROTEIN WAS CRYSTALLIZED FROM SODIUM ACETATE BUFFER (PH 4.3) CONTAINING 6.0% (W/V) SODIUM CHLORIDE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 50 (mg/ml) | |
2 | 1 | drop | HAc-NaAc | 10 (mM) | |
3 | 1 | reservoir | HAc-NaAc | 0.2 (M) | |
4 | 1 | reservoir | 6.0 (%(w/v)) |