2KZM
KLENOW FRAGMENT WITH NORMAL SUBSTRATE AND ZINC AND MANGANESE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-06-15 |
| Detector | ADSC |
| Spacegroup name | P 43 |
| Unit cell lengths | 102.600, 102.600, 86.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.24200 * |
| Structure solution method | RIGID-BODY REFINEMENT |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 1.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.055 * | 0.211 * |
| Number of reflections | 22445 * | |
| <I/σ(I)> | 17.3 | 5.2 |
| Completeness [%] | 89.1 * | 73.9 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 * | 18 * | Brick, P., (1983) J. Mol. Biol., 166, 453. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM CITRATE | ||
| 2 | 1 | 2 | SODIUM CITRATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1 (%) | |
| 2 | 1 | drop | sodium citrate | 1.1-1.2 (M) |
