2KFZ
KLENOW FRAGMENT WITH BRIDGING-SULFUR SUBSTRATE AND ZINC ONLY
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1997-06-15 |
Detector | ADSC |
Spacegroup name | P 43 |
Unit cell lengths | 102.900, 102.900, 86.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.030 |
R-factor | 0.225 * |
Rwork | 0.215 |
R-free | 0.25500 |
Structure solution method | RIGID-BODY REFINEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 23.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 2.030 | 2.030 |
Rmerge | 0.063 * | 0.332 * |
Total number of observations | 422599 * | |
Number of reflections | 59988 | |
<I/σ(I)> | 16.3 | 3.5 |
Completeness [%] | 98.5 | 97.6 |
Redundancy | 4.5 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 * | 18 * | Brick, P., (1983) J. Mol. Biol., 166, 453. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | SODIUM CITRATE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein solution | 1 (%) | |
2 | 1 | drop | sodium citrate | 1.1-1.2 (M) |