2JLJ
Crystal Structure of the cytoplasmic domain of Yersinia pestis YscU N263A P264A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-05 |
Detector | MARRESEARCH |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 66.041, 66.041, 70.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.280 - 1.300 |
R-factor | 0.22 |
Rwork | 0.219 |
R-free | 0.24800 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.017 |
RMSD bond angle | 1.701 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.4.0057) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.340 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.080 | 0.590 |
Number of reflections | 37461 | |
<I/σ(I)> | 19.2 | 1.6 |
Completeness [%] | 95.5 | 66.5 |
Redundancy | 8.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 100 MM HEPES PH 7, 1 M SODIUM MALONATE PH 7, 0.75 M SODIUM CHLORIDE |