2JFY
Crystal structure of Helicobacter pylori glutamate racemase in complex with D-Glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.280, 78.960, 59.140 |
| Unit cell angles | 90.00, 92.64, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.900 |
| Rwork | 0.206 |
| R-free | 0.24580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jfx |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.234 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNX (2000.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.340 |
| Number of reflections | 34987 | |
| <I/σ(I)> | 8.5 | 2.1 |
| Completeness [%] | 94.4 | 78.6 |
| Redundancy | 4.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PROTEIN FORMULATED AT 10 MG/ML WITH 200 MM AMMONIUM ACETATE PH 7.4, 5 MM D-L GLUTAMATE, 1 MM TCEP AND CRYSTALLISED WITH 100 MM TRIS PH 8.5, 200 MM MGSO4, AND 25% PEG4000 |
