2JE1
The crystal Structure of the tumor supressor protein pp32 (Anp32a) :structural insights into the Anp32 family of proteins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-12 |
Detector | ADSC CCD |
Spacegroup name | P 63 |
Unit cell lengths | 106.506, 106.506, 133.153 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 2.690 |
R-factor | 0.241 |
Rwork | 0.238 |
R-free | 0.30300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | HOMOLOGY MODEL |
RMSD bond length | 0.011 |
RMSD bond angle | 1.485 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 25.000 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.130 | 0.540 |
Number of reflections | 23193 | |
<I/σ(I)> | 11.1 | 3.1 |
Completeness [%] | 97.8 | 99.3 |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | pH 5.50 |