2IZT
Structure of casein kinase gamma 3 in complex with inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-04-10 |
Detector | RIGAKU IMAGE PLATE |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 56.433, 56.433, 220.266 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.350 - 2.000 |
R-factor | 0.225 |
Rwork | 0.222 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2chl |
RMSD bond length | 0.014 |
RMSD bond angle | 1.527 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.700 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.090 | 0.360 |
Number of reflections | 26655 | |
<I/σ(I)> | 10.9 | 2.4 |
Completeness [%] | 93.0 | 87.6 |
Redundancy | 4.3 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 0.05 M CACL2, 20% PEG6000, 10% ETHYLENE GLYCOL, 0.10 M TRIS PH=8.0, pH 8.00 |