2IY9
Crystal structure of the A-subunit of the AB5 toxin from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-07-27 |
Detector | RAXIS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.967, 78.987, 95.782 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mee |
RMSD bond length | 0.009 |
RMSD bond angle | 1.199 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.050 | 0.150 |
Number of reflections | 31757 | |
<I/σ(I)> | 15 | 2 |
Completeness [%] | 99.0 | 88 |
Redundancy | 2.1 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |