2IVD
Structure of protoporphyrinogen oxidase from Myxococcus xanthus with acifluorfen
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 148.918, 148.918, 131.924 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.390 - 2.300 |
R-factor | 0.235 |
Rwork | 0.234 |
R-free | 0.28300 |
Structure solution method | OTHER |
RMSD bond length | 0.010 |
RMSD bond angle | 1.487 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.400 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.070 | 0.530 |
Number of reflections | 55419 | |
<I/σ(I)> | 8.7 | 1 |
Completeness [%] | 75.2 | 46.7 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1M TRIS/HCL PH 7.5, 1.5M AMMONIUM SULPHATE, 20% GLYCEROL, 1% PEG 4000 |