2I91
60kDa Ro autoantigen in complex with a fragment of misfolded RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2005-04-25 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.680, 119.980, 73.540 |
Unit cell angles | 90.00, 98.91, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.650 |
R-factor | 0.245 |
Rwork | 0.241 |
R-free | 0.28500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yvp |
RMSD bond length | 0.009 |
RMSD bond angle | 1.626 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.740 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.130 | 0.398 |
Number of reflections | 34410 | |
<I/σ(I)> | 12 | 3.9 |
Completeness [%] | 96.5 | 99.8 |
Redundancy | 3.3 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 18-20% PEG 6000, 250 mM magnesium acetate, 100 mM sodium acetate Protein was mixed with RNA approximately 1:1., pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 6000 | ||
2 | 1 | 1 | magnesium acetate | ||
3 | 1 | 1 | sodium acetate | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | PEG 6000 | ||
6 | 1 | 2 | magnesium acetate | ||
7 | 1 | 2 | sodium acetate |