2I2O

Crystal Structure of an eIF4G-like Protein from Danio rerio

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-04
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97925
Spacegroup name	C 1 2 1
Unit cell lengths	317.796, 40.948, 40.917
Unit cell angles	90.00, 90.26, 90.00

Refinement procedure

Resolution	40.612 - 1.920
R-factor	0.192
Rwork	0.190
R-free	0.23500
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.357
Data reduction software	HKL-2000
Data scaling software	HKL
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.612	40.610	1.990
High resolution limit [Å]	1.920	4.140	1.920
Rmerge	0.074	0.054	0.245
Number of reflections	39789
<I/σ(I)>	13.377		5.769
Completeness [%]	97.5	100	95.5
Redundancy	6.4	7.1	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PROTEIN SOLUTION (10 MG/ML PROTEIN, 0.050 M SODIUM CHLORIDE, 0.003 M SODIUM AZIDE, 0.0003 M TCEP, 0.005 M BisTris PH 7.0) MIXED IN A 1:1 RATIO WITH THE WELL SOLUTION (7% PEG 4K, 0.40 M sodium chloride, 0.1 M MES/Acetate pH 5.5) CRYOPROTECTED WITH 10% PEG 4K, 0.1 M MES/Acetate pH 5.5, 25% Ethylene glycol, vapor diffusion, hanging drop, temperature 293K