2HYF
The Structure of apo-MntR from Bacillus subtilis, selenomethionine derivative
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9793,0.9796,0.9641 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.890, 65.030, 186.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| Rwork | 0.217 |
| R-free | 0.27700 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.600 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.106 | 0.383 |
| Number of reflections | 16290 | |
| <I/σ(I)> | 6.3 | 2 |
| Completeness [%] | 93.6 | 79.5 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 15% PEG 400, 0.2 M ammonium sulfate, 5 mM cobalt(II) chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
