2HXU
Crystal structure of K220A mutant of L-Fuconate Dehydratase from Xanthomonas campestris liganded with Mg++ and L-fuconate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-16 |
Detector | ADSC |
Wavelength(s) | 0.97911 |
Spacegroup name | P 6 2 2 |
Unit cell lengths | 159.154, 159.154, 101.961 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.600 - 1.800 |
R-factor | 0.184 |
Rwork | 0.172 |
R-free | 0.18300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hne |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.600 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 67796 | |
Completeness [%] | 96.1 | 89.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2.0 M ammonium sulfate, 0.1M Hepes , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |