2HLP
CRYSTAL STRUCTURE OF THE E267R MUTANT OF A HALOPHILIC MALATE DEHYDROGENASE IN THE APO FORM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 279 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 114.160, 129.980, 123.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.000 - 2.590 |
Rwork | 0.194 |
R-free | 0.23200 * |
Structure solution method | OTHER |
Starting model (for MR) | 1hlp |
RMSD bond length | 0.009 * |
RMSD bond angle | 1.500 * |
Data reduction software | XDS |
Data scaling software | CCP4 |
Refinement software | CNS (0.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.000 | 2.750 |
High resolution limit [Å] | 2.590 | 2.590 |
Rmerge | 0.086 * | 0.271 |
Total number of observations | 159628 * | |
Number of reflections | 26445 | |
<I/σ(I)> | 7.3 | 2.2 |
Completeness [%] | 88.5 * | 71.3 * |
Redundancy | 3.8 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.6 | 6 * | 1.7M NACL, 20MM TRIS, PH 7.6, 57% MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 14 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | |
3 | 1 | drop | 1.8 (M) | ||
4 | 1 | drop | MPD | 58 (%) | |
5 | 1 | reservoir | MPD | 60-70 (%(v/v)) |