2HJW

Crystal Structure of the BC domain of ACC2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-05
Detector	ADSC QUANTUM
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	75.797, 75.797, 189.006
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.860 - 2.500
R-factor	0.212
Rwork	0.212
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w93
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.580
High resolution limit [Å]	2.500	2.500
Number of reflections	20983
Completeness [%]	92.4	70.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	HANGING DROP	6.5	293	AMMONIUM SULFATE, MES, pH 6.5, HANGING DROP, temperature 293K