2HC9
Structure of Caenorhabditis elegans leucine aminopeptidase-zinc complex (LAP1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 131.309, 131.309, 126.344 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.14276 |
| Rwork | 0.142 |
| R-free | 0.17526 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hb6 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.364 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.069 | 0.461 |
| Number of reflections | 55114 | |
| <I/σ(I)> | 8.9 | 1.9 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 11.3 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 290 | 100 MM TRIS, 2 M AMMONIUM SULFATE, PH 8.5, 10% GLYCEROL, 200 MM SODIUM CHLORIDE, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K |
