2H0G
Crystal Structure of DsbG T200M mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9724 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.226, 56.829, 85.794 |
| Unit cell angles | 90.00, 95.56, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| Rwork | 0.227 |
| R-free | 0.27000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1v57 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | MX (SYSTEM) |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.063 | 0.344 |
| Number of reflections | 25647 | |
| <I/σ(I)> | 20 | 3 |
| Completeness [%] | 97.2 | 87.2 |
| Redundancy | 4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.9 | 298 | 18% PEG 4000, 0.1M sodium citrate, 0.2M ammonium sulfate, pH 3.9, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
