2GRK
Crystal structure of ectromelia virus EVM1 chemokine binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-05-01 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.200, 55.800, 85.800 |
Unit cell angles | 90.00, 106.30, 90.00 |
Refinement procedure
Resolution | 19.880 - 2.600 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cq3 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.112 | 0.426 |
Number of reflections | 13573 | |
<I/σ(I)> | 9.547 | 2.36 |
Completeness [%] | 97.6 | 96.4 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.2 | 293 | 2 M ammonium sulfate, 3% ethylene glycol, 0.1 M cacodylate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K, pH 6.20 |