2GFY
Structure of E. coli FabF(K335A) mutant with covalently linked dodecanoic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 74.730, 74.730, 148.072 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 64.550 - 2.850 |
R-factor | 0.1691 |
Rwork | 0.166 |
R-free | 0.24290 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.372 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | BUSTER-TNT (1.3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.600 | 3.000 |
High resolution limit [Å] | 2.850 | 2.850 |
Number of reflections | 11514 | |
Completeness [%] | 98.2 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 19-24% PEG 8000, 0.1M Tris, 10mM BME, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |