2GD5
Structural basis for budding by the ESCRTIII factor CHMP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-31 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.97927 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.580, 72.570, 89.230 |
| Unit cell angles | 68.68, 83.61, 76.70 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.26106 |
| Rwork | 0.259 |
| R-free | 0.30106 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.511 |
| Data scaling software | XDS |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 3.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.096 | 0.464 |
| Number of reflections | 17853 | |
| <I/σ(I)> | 14.33 | 4.98 |
| Completeness [%] | 94.7 | 94.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 8-12% PEG 3350, 200mM ammonium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
