2GCG
Ternary Crystal Structure of Human Glyoxylate Reductase/Hydroxypyruvate Reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.568, 66.877, 149.780 |
| Unit cell angles | 90.00, 98.22, 90.00 |
Refinement procedure
| Resolution | 46.200 - 2.200 |
| R-factor | 0.205 |
| Rwork | 0.201 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gdh |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.857 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (V. 1.3) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.121 | 0.275 |
| Number of reflections | 69070 | 5483 |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 90.3 | 72.3 |
| Redundancy | 2.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 15% PEG 8000, 0.2M ammonium sulfate, 0.1M sodium cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
