2GC7
Substrate reduced, copper free complex of methylamine dehydrogenase, amicyanin and cytochrome c551i from Paracoccus denitrificans.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 298 |
Detector technology | DIFFRACTOMETER |
Collection date | 1996-05-25 |
Detector | WEISSENBERG |
Wavelength(s) | 0.95 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.323, 189.127, 128.906 |
Unit cell angles | 90.00, 99.75, 90.00 |
Refinement procedure
Resolution | 29.940 - 1.900 |
Rwork | 0.171 |
R-free | 0.19800 |
Structure solution method | Refined directly |
Starting model (for MR) | 2gc4 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.055 | 0.276 |
Number of reflections | 254385 | |
<I/σ(I)> | 12.4 | 2.2 |
Completeness [%] | 83.2 | 44.8 |
Redundancy | 1.8 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 2.3-2.6M sodium/potassium phosphate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |