2GAL
CRYSTAL STRUCTURE OF HUMAN GALECTIN-7 IN COMPLEX WITH GALACTOSE
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 289 |
Detector technology | IMAGE PLATE |
Collection date | 1997-08 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.470, 64.520, 72.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | FREE GALECTIN-7 |
RMSD bond length | 0.008 |
RMSD bond angle | 30.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.077 | |
Total number of observations | 172647 * | |
Number of reflections | 18155 | |
<I/σ(I)> | 9 | 6.6 |
Completeness [%] | 98.2 | 97.4 |
Redundancy | 9.5 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 16 * | CRYSTALS WERE GROWN USING THE HANGING DROP METHOD FROM DROPS CONTAINING 9 MG/ML PROTEIN AT PH 8.1 IN 50 MM SODIUM PHOSPHATE BUFFER, 0.3 M SODIUM CHLORIDE, 20 MM IMIDAZOLE, 8.5% PEG 3350 AND 25 MM GALACTOSE. DROPS WERE EQUILIBRATED AGAINST RESERVOIRS CONTAINING 50 MM SODIUM PHOSPHATE BUFFER, 0.3 M SODIUM CHLORIDE, 20 MM IMIDAZOLE, 17% PEG 3350 AND 50 MM GALACTOSE., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9 (mg/ml) | |
2 | 1 | drop | sodium phosphate | 50 (mM) | |
3 | 1 | drop | 0.3 (M) | ||
4 | 1 | drop | imidazol | 20 (mM) | |
5 | 1 | drop | PEG3350 | 8.5 (%) | |
6 | 1 | reservoir | sodium phosphate | 50 (mM) | |
7 | 1 | reservoir | 0.3 (M) | ||
8 | 1 | reservoir | imidazole | 20 (mM) | |
9 | 1 | reservoir | PEG3350 | 17 (%) |