2FRX
Crystal structure of YebU, a m5C RNA methyltransferase from E.coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-19 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.962 |
Spacegroup name | P 1 |
Unit cell lengths | 67.706, 87.126, 95.048 |
Unit cell angles | 88.33, 76.79, 90.19 |
Refinement procedure
Resolution | 29.030 - 2.900 |
R-factor | 0.234 |
Rwork | 0.231 |
R-free | 0.28200 |
Structure solution method | SAD |
RMSD bond length | 0.022 |
RMSD bond angle | 1.758 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 45870 | |
<I/σ(I)> | 9.13 | 2.14 |
Completeness [%] | 97.9 | 97.5 |
Redundancy | 3.22 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 15%(w/v) PEG 8000, 50mM mono-potassium dihydrogen phosphate, 10mM BaCl2 or 15% PEG 5000 MME, sodium acetate pH 4.6, 0.2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |