2FQ4
The crystal structure of the transcriptional regulator (TetR family) from Bacillus cereus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.891, 67.648, 111.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.640 - 1.790 |
| R-factor | 0.23683 |
| Rwork | 0.235 |
| R-free | 0.26208 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.311 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.640 | 1.841 |
| High resolution limit [Å] | 1.790 | 1.795 |
| Rmerge | 0.059 | 0.680 |
| Number of reflections | 17099 | |
| <I/σ(I)> | 40.5 | 1.45 |
| Completeness [%] | 94.7 | 80 |
| Redundancy | 12.2 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 25% PEG3350, 0.2M NaCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
