2FCY
HIV-1 DIS kissing-loop in complex with Neomycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.91946 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 27.105, 113.702, 96.165 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.220 - 2.200 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fcx |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 7689 | |
| <I/σ(I)> | 20.7 | 6.4 |
| Completeness [%] | 96.7 | 90.2 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 310 | MPD, KCl, MgCl2, Na cacodylate pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 310K |
