2FCX
HIV-1 DIS kissing-loop in complex with neamine
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.91961 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 27.005, 113.395, 95.038 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.420 - 2.000 |
R-factor | 0.267 |
Rwork | 0.267 |
R-free | 0.29200 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 18852 | |
<I/σ(I)> | 24.4 | 4 |
Completeness [%] | 98.6 | 92.7 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 310 | MPD, KCl, MgCl2, Na Cacodylate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 310K |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 310 | MPD, KCl, MgCl2, Na Cacodylate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 310K |