2FA9
The crystal structure of Sar1[H79G]-GDP provides insight into the coat-controlled GTP hydrolysis in the disassembly of COP II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-11 |
Detector | ENRAF-NONIUS |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.215, 61.620, 71.165 |
Unit cell angles | 90.00, 72.75, 90.00 |
Refinement procedure
Resolution | 45.650 - 2.500 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.28900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f6b |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | PROTEUM PLUS |
Data scaling software | LSCALE |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.000 | |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 13619 | |
Completeness [%] | 88.4 | 88.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION, RECRYSTALLIZATION | 5.8 | 298 | 28%(w/v)Polyethylene Glycol-4000, 100mM sodium acetate, 0.2M ammonium sulfate , pH 5.8, EVAPORATION, RECRYSTALLIZATION, temperature 298K |