2F8S
Crystal structure of Aa-Ago with externally-bound siRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-20 |
Detector | MARRESEARCH |
Wavelength(s) | 1.1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.697, 117.516, 98.620 |
Unit cell angles | 90.00, 99.12, 90.00 |
Refinement procedure
Resolution | 40.000 - 3.000 |
R-factor | 0.21717 |
Rwork | 0.212 |
R-free | 0.30736 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Aa-Ago pdb entry 1YVU |
RMSD bond length | 0.015 |
RMSD bond angle | 1.699 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.051 | 0.304 |
Number of reflections | 38034 | |
Completeness [%] | 1.0 | 0.913 |
Redundancy | 6.9 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG 3350, 25mM NH4F , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG 3350, 25mM NH4F , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 3350 | ||
2 | 1 | 1 | NH4F | ||
3 | 1 | 1 | H2O | ||
4 | 1 | 2 | PEG 3350 | ||
5 | 1 | 2 | NH4F | ||
6 | 1 | 2 | H2O |